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biotinylated rna  (Thermo Fisher)


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    Structured Review

    Thermo Fisher biotinylated rna
    Biotinylated Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+rna/Streptavidin/bio_rxiv__2025__11__29__691298-197-1-19
    Average 99 stars, based on 1 article reviews
    biotinylated rna - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Magnetic Beads:

    Article Title: LARP4B inhibits ferroptosis and accelerates the progression of pancreatic cancer by activating WNK1-induced NRF2/GCH1/BH4 pathway.
    Article Snippet: Transcribed RNAs were subsequently biotin-labeled with the Biotin RNA Labeling Mix (Thermo Scientific, 20 163). .. Next, 100 pmol biotinylated RNA was chemically coupled to streptavidin-linked magnetic beads (75 μL) at room temperature for 2 h (Thermo Scientific, 20 164). ..

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma.
    Article Snippet: RNA Pull-Down Assay: The HMGCS1 RNA probes (50 pmol) were transcribed in vitro using the T7 In Vitro Transcription Kit, and then, biotin RNA was used to label HMGCS1 mRNA and its truncations according to the manufacturer’s protocol (20 163, Thermo). .. Then, 100 pmol of biotinylated RNA was chemically coupled to streptavidin-conjugated magnetic beads (50 μL) for 2 h at room temperature (20 164, Thermo). ..

    Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF‐1α by Promoting Cholesterol Production in Hepatocellular Carcinoma
    Article Snippet: The HMGCS1 RNA probes (50 pmol) were transcribed in vitro using the T7 In Vitro Transcription Kit, and then, biotin RNA was used to label HMGCS1 mRNA and its truncations according to the manufacturer's protocol (20 163, Thermo). .. Then, 100 pmol of biotinylated RNA was chemically coupled to streptavidin‐conjugated magnetic beads (50 μL) for 2 h at room temperature (20 164, Thermo). ..

    Article Title: RECQ5 mediates pre-rRNA processing in nucleolus
    Article Snippet: HeLa cells were pretreated with 20 μM 5-fluorouracil (5-FU, Sigma) for 3 h to arrest pre-rRNA processing and then pulse-chased with 100 μM 4-thiouridine (4sU, Sigma) for the final 30 min to monitor nascent pre-rRNA [ ]. .. Total RNA was extracted and biotinylated in biotinylation buffer (10 mM Tris, pH 7.5, 1 mM EDTA) with 0.2 mg/ml EZ-Link Biotin-HPDP (Pierce) at room temperature for 1.5 h. Biotinylated RNA was incubated with streptavidin magnetic beads (Invitrogen) at room temperature for 30 min. ..

    Incubation:

    Article Title: Peli1, regulated by m 6 A modification, suppresses NLRP3 inflammasome activation in atherosclerosis by inhibiting YB-1
    Article Snippet: Approximately 2 × 10 7 cells were dissolved in the soft lysis buffer plus 80 U/mL RNasin (Promega, Madison, WI, USA). .. The cell extract was incubated with biotinylated RNA for 1 h. Washed streptavidin-coupled agarose beads (Invitrogen) were added to each binding reaction and further incubated for 1 h. Beads were subsequently washed six times in the lysis buffer. ..

    Article Title: RECQ5 mediates pre-rRNA processing in nucleolus
    Article Snippet: HeLa cells were pretreated with 20 μM 5-fluorouracil (5-FU, Sigma) for 3 h to arrest pre-rRNA processing and then pulse-chased with 100 μM 4-thiouridine (4sU, Sigma) for the final 30 min to monitor nascent pre-rRNA [ ]. .. Total RNA was extracted and biotinylated in biotinylation buffer (10 mM Tris, pH 7.5, 1 mM EDTA) with 0.2 mg/ml EZ-Link Biotin-HPDP (Pierce) at room temperature for 1.5 h. Biotinylated RNA was incubated with streptavidin magnetic beads (Invitrogen) at room temperature for 30 min. ..

    Binding Assay:

    Article Title: Peli1, regulated by m 6 A modification, suppresses NLRP3 inflammasome activation in atherosclerosis by inhibiting YB-1
    Article Snippet: Approximately 2 × 10 7 cells were dissolved in the soft lysis buffer plus 80 U/mL RNasin (Promega, Madison, WI, USA). .. The cell extract was incubated with biotinylated RNA for 1 h. Washed streptavidin-coupled agarose beads (Invitrogen) were added to each binding reaction and further incubated for 1 h. Beads were subsequently washed six times in the lysis buffer. ..

    Article Title: Generative inverse design of RNA structure and function with gRNAde
    Article Snippet: .. The biotinylated RNA was isolated from the reactions by binding to streptavidin coated paramagnetic beads (Dynabeads MyOne Streptavidin C1, Invitrogen). ..

    Lysis:

    Article Title: Peli1, regulated by m 6 A modification, suppresses NLRP3 inflammasome activation in atherosclerosis by inhibiting YB-1
    Article Snippet: Approximately 2 × 10 7 cells were dissolved in the soft lysis buffer plus 80 U/mL RNasin (Promega, Madison, WI, USA). .. The cell extract was incubated with biotinylated RNA for 1 h. Washed streptavidin-coupled agarose beads (Invitrogen) were added to each binding reaction and further incubated for 1 h. Beads were subsequently washed six times in the lysis buffer. ..

    Isolation:

    Article Title: Generative inverse design of RNA structure and function with gRNAde
    Article Snippet: .. The biotinylated RNA was isolated from the reactions by binding to streptavidin coated paramagnetic beads (Dynabeads MyOne Streptavidin C1, Invitrogen). ..

    Reverse Transcription:

    Article Title: Global mapping of RNA N 6 -methyladenosine (m 6 A) in human subcutaneous and visceral adipose tissue reveals novel targets that correlate with clinical variables of obesity.
    Article Snippet: .. Biotinylated RNA was pulled down with streptavidin beads, and 500 ng RNA was used as input for reverse transcription using SuperScriptTM VILOTM cDNA Synthesis Kit (Thermo Fisher Scientific). .. The relative amount of biotinylated transcripts were quantified by qPCR on a QuantStudio Flex 7 system (Thermo Fisher Scientific) using PowerUP SYBR green mastermix (Thermo Fisher Scientific) with GAPDH and ACTB as reference genes.

    Article Title: Global mapping of RNA N 6 -methyladenosine (m 6 A) in human subcutaneous and visceral adipose tissue reveals novel targets that correlate with clinical variables of obesity
    Article Snippet: .. Biotinylated RNA was pulled down with streptavidin beads, and 500 ng RNA was used as input for reverse transcription using SuperScriptTM VILOTM cDNA Synthesis Kit (Thermo Fisher Scientific). .. The relative amount of biotinylated transcripts were quantified by qPCR on a QuantStudio Flex 7 system (Thermo Fisher Scientific) using PowerUP SYBR green mastermix (Thermo Fisher Scientific) with GAPDH and ACTB as reference genes.

    cDNA Synthesis:

    Article Title: Global mapping of RNA N 6 -methyladenosine (m 6 A) in human subcutaneous and visceral adipose tissue reveals novel targets that correlate with clinical variables of obesity.
    Article Snippet: .. Biotinylated RNA was pulled down with streptavidin beads, and 500 ng RNA was used as input for reverse transcription using SuperScriptTM VILOTM cDNA Synthesis Kit (Thermo Fisher Scientific). .. The relative amount of biotinylated transcripts were quantified by qPCR on a QuantStudio Flex 7 system (Thermo Fisher Scientific) using PowerUP SYBR green mastermix (Thermo Fisher Scientific) with GAPDH and ACTB as reference genes.

    Article Title: Global mapping of RNA N 6 -methyladenosine (m 6 A) in human subcutaneous and visceral adipose tissue reveals novel targets that correlate with clinical variables of obesity
    Article Snippet: .. Biotinylated RNA was pulled down with streptavidin beads, and 500 ng RNA was used as input for reverse transcription using SuperScriptTM VILOTM cDNA Synthesis Kit (Thermo Fisher Scientific). .. The relative amount of biotinylated transcripts were quantified by qPCR on a QuantStudio Flex 7 system (Thermo Fisher Scientific) using PowerUP SYBR green mastermix (Thermo Fisher Scientific) with GAPDH and ACTB as reference genes.



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    Miltenyi Biotec biotinylated rna
    Transient transcriptome sequencing can capture eRNAs genome-wide (a) Schematic representing the workflow of TT-seq. Nascent <t>RNA</t> is metabolically labelled in vivo via nucleoside analogue, 4-thiouridine (4sU). Total RNA is extracted and fragmented. 4sU-labelled fragmented RNA is <t>biotinylated</t> and captured using steptavidin pull-down prior to library preparation and sequencing ( ; ). (b) Log-transformed gene counts of WT SH-SY5Y TT-seq data from two replicates both prior and post spike-in normalisation. (c) Correlation of spike-in normalised gene counts between WT SH-SY5Y TT-seq replicates. Correlation strength quantified by the Pearson correlation coefficient. (d) Exonic to intronic per gene transcript ratios for TT-seq in SH-SY5Y cells, compared to total RNA-seq in SH-SY5Y cells , to recapitulate the expected values for TT-seq, with the coverage of intronic to exonic transcripts per gene estimated to be ∼ 60% . (e) Proportion of total TT-seq reads in SH-SY5Y cells that map to non-coding regions of the genome, compared to the corresponding proportions observed in total RNA-seq data from the same cell type. (f) WT SH-SY5Y TT-seq coverage ( log 2 CPM+1) over ABC candidate enhancers compared to shuffled control regions. p -values determined by Wilcoxon Rank Sum. (g) Number of ALS-associated SNPs transcribed versus untranscribed candidate CAV1/CAV2 enhancers ( ; ), as determined by TT-seq in WT SH-SY5Y cells. p -value deteremined by Wilcoxon Rank Sum.
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    Image Search Results


    Transient transcriptome sequencing can capture eRNAs genome-wide (a) Schematic representing the workflow of TT-seq. Nascent RNA is metabolically labelled in vivo via nucleoside analogue, 4-thiouridine (4sU). Total RNA is extracted and fragmented. 4sU-labelled fragmented RNA is biotinylated and captured using steptavidin pull-down prior to library preparation and sequencing ( ; ). (b) Log-transformed gene counts of WT SH-SY5Y TT-seq data from two replicates both prior and post spike-in normalisation. (c) Correlation of spike-in normalised gene counts between WT SH-SY5Y TT-seq replicates. Correlation strength quantified by the Pearson correlation coefficient. (d) Exonic to intronic per gene transcript ratios for TT-seq in SH-SY5Y cells, compared to total RNA-seq in SH-SY5Y cells , to recapitulate the expected values for TT-seq, with the coverage of intronic to exonic transcripts per gene estimated to be ∼ 60% . (e) Proportion of total TT-seq reads in SH-SY5Y cells that map to non-coding regions of the genome, compared to the corresponding proportions observed in total RNA-seq data from the same cell type. (f) WT SH-SY5Y TT-seq coverage ( log 2 CPM+1) over ABC candidate enhancers compared to shuffled control regions. p -values determined by Wilcoxon Rank Sum. (g) Number of ALS-associated SNPs transcribed versus untranscribed candidate CAV1/CAV2 enhancers ( ; ), as determined by TT-seq in WT SH-SY5Y cells. p -value deteremined by Wilcoxon Rank Sum.

    Journal: bioRxiv

    Article Title: Amyotrophic Lateral Sclerosis-associated 3′ UTR enhancer embedded within CAV1 risk gene

    doi: 10.1101/2025.09.09.675029

    Figure Lengend Snippet: Transient transcriptome sequencing can capture eRNAs genome-wide (a) Schematic representing the workflow of TT-seq. Nascent RNA is metabolically labelled in vivo via nucleoside analogue, 4-thiouridine (4sU). Total RNA is extracted and fragmented. 4sU-labelled fragmented RNA is biotinylated and captured using steptavidin pull-down prior to library preparation and sequencing ( ; ). (b) Log-transformed gene counts of WT SH-SY5Y TT-seq data from two replicates both prior and post spike-in normalisation. (c) Correlation of spike-in normalised gene counts between WT SH-SY5Y TT-seq replicates. Correlation strength quantified by the Pearson correlation coefficient. (d) Exonic to intronic per gene transcript ratios for TT-seq in SH-SY5Y cells, compared to total RNA-seq in SH-SY5Y cells , to recapitulate the expected values for TT-seq, with the coverage of intronic to exonic transcripts per gene estimated to be ∼ 60% . (e) Proportion of total TT-seq reads in SH-SY5Y cells that map to non-coding regions of the genome, compared to the corresponding proportions observed in total RNA-seq data from the same cell type. (f) WT SH-SY5Y TT-seq coverage ( log 2 CPM+1) over ABC candidate enhancers compared to shuffled control regions. p -values determined by Wilcoxon Rank Sum. (g) Number of ALS-associated SNPs transcribed versus untranscribed candidate CAV1/CAV2 enhancers ( ; ), as determined by TT-seq in WT SH-SY5Y cells. p -value deteremined by Wilcoxon Rank Sum.

    Article Snippet: Biotinylated RNA was enriched by streptavidin pull-down using μ MACS Streptavidin MicroBeads (Miltenyi Biotec) and eluted in RNase-free water.

    Techniques: Sequencing, Genome Wide, Metabolic Labelling, In Vivo, Transformation Assay, RNA Sequencing, Control