biotinylated rna (Thermo Fisher)
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Biotinylated Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+rna/Streptavidin/bio_rxiv__2025__11__29__691298-197-1-19
Average 99 stars, based on 1 article reviews
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Magnetic Beads:Article Title: LARP4B inhibits ferroptosis and accelerates the progression of pancreatic cancer by activating WNK1-induced NRF2/GCH1/BH4 pathway. Article Snippet: Transcribed RNAs were subsequently biotin-labeled with the Biotin RNA Labeling Mix (Thermo Scientific, 20 163). .. Next, 100 pmol Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF-1α by Promoting Cholesterol Production in Hepatocellular Carcinoma. Article Snippet: RNA Pull-Down Assay: The HMGCS1 RNA probes (50 pmol) were transcribed in vitro using the T7 In Vitro Transcription Kit, and then, biotin RNA was used to label HMGCS1 mRNA and its truncations according to the manufacturer’s protocol (20 163, Thermo). .. Then, 100 pmol of Article Title: RPL6 Interacts with HMGCS1 to Stabilize HIF‐1α by Promoting Cholesterol Production in Hepatocellular Carcinoma Article Snippet: The HMGCS1 RNA probes (50 pmol) were transcribed in vitro using the T7 In Vitro Transcription Kit, and then, biotin RNA was used to label HMGCS1 mRNA and its truncations according to the manufacturer's protocol (20 163, Thermo). .. Then, 100 pmol of Article Title: RECQ5 mediates pre-rRNA processing in nucleolus Article Snippet: HeLa cells were pretreated with 20 μM 5-fluorouracil (5-FU, Sigma) for 3 h to arrest pre-rRNA processing and then pulse-chased with 100 μM 4-thiouridine (4sU, Sigma) for the final 30 min to monitor nascent pre-rRNA [ ]. .. Total RNA was extracted and biotinylated in biotinylation buffer (10 mM Tris, pH 7.5, 1 mM EDTA) with 0.2 mg/ml EZ-Link Biotin-HPDP (Pierce) at room temperature for 1.5 h. Incubation:Article Title: Peli1, regulated by m 6 A modification, suppresses NLRP3 inflammasome activation in atherosclerosis by inhibiting YB-1 Article Snippet: Approximately 2 × 10 7 cells were dissolved in the soft lysis buffer plus 80 U/mL RNasin (Promega, Madison, WI, USA). .. The cell extract was incubated with Article Title: RECQ5 mediates pre-rRNA processing in nucleolus Article Snippet: HeLa cells were pretreated with 20 μM 5-fluorouracil (5-FU, Sigma) for 3 h to arrest pre-rRNA processing and then pulse-chased with 100 μM 4-thiouridine (4sU, Sigma) for the final 30 min to monitor nascent pre-rRNA [ ]. .. Total RNA was extracted and biotinylated in biotinylation buffer (10 mM Tris, pH 7.5, 1 mM EDTA) with 0.2 mg/ml EZ-Link Biotin-HPDP (Pierce) at room temperature for 1.5 h. Binding Assay:Article Title: Peli1, regulated by m 6 A modification, suppresses NLRP3 inflammasome activation in atherosclerosis by inhibiting YB-1 Article Snippet: Approximately 2 × 10 7 cells were dissolved in the soft lysis buffer plus 80 U/mL RNasin (Promega, Madison, WI, USA). .. The cell extract was incubated with Article Title: Generative inverse design of RNA structure and function with gRNAde Article Snippet: .. The Lysis:Article Title: Peli1, regulated by m 6 A modification, suppresses NLRP3 inflammasome activation in atherosclerosis by inhibiting YB-1 Article Snippet: Approximately 2 × 10 7 cells were dissolved in the soft lysis buffer plus 80 U/mL RNasin (Promega, Madison, WI, USA). .. The cell extract was incubated with Isolation:Article Title: Generative inverse design of RNA structure and function with gRNAde Article Snippet: .. The Reverse Transcription:Article Title: Global mapping of RNA N 6 -methyladenosine (m 6 A) in human subcutaneous and visceral adipose tissue reveals novel targets that correlate with clinical variables of obesity. Article Snippet: .. Article Title: Global mapping of RNA N 6 -methyladenosine (m 6 A) in human subcutaneous and visceral adipose tissue reveals novel targets that correlate with clinical variables of obesity Article Snippet: .. cDNA Synthesis:Article Title: Global mapping of RNA N 6 -methyladenosine (m 6 A) in human subcutaneous and visceral adipose tissue reveals novel targets that correlate with clinical variables of obesity. Article Snippet: .. Article Title: Global mapping of RNA N 6 -methyladenosine (m 6 A) in human subcutaneous and visceral adipose tissue reveals novel targets that correlate with clinical variables of obesity Article Snippet: .. |
